A molecular atlas of cell types and zonation in the brain vasculature

Cerebrovascular disease is the third most common cause of death in developed countries, but our understanding of the cells that compose the cerebral vasculature is limited. Here, using vascular single-cell transcriptomics, we provide molecular definitions for the principal types of blood vascular and vessel-associated cells in the adult mouse brain. We uncover the transcriptional basis of the gradual phenotypic change (zonation) along the arteriovenous axis and reveal unexpected cell type differences: a seamless continuum for endothelial cells versus a punctuated continuum for mural cells. We also provide insight into pericyte organotypicity and define a population of perivascular fibroblast-like cells that are present on all vessel types except capillaries. Our work illustrates the power of single-cell transcriptomics to decode the higher organizational principles of a tissue and may provide the initial chapter in a molecular encyclopaedia of the mammalian vasculature.Peer reviewe

Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling.

Laser capture microscopy (LCM) coupled with global transcriptome profiling could enable precise analyses of cell populations without the need for tissue dissociation, but has so far required relatively large numbers of cells. Here we report a robust and highly efficient strategy for LCM coupled with full-length mRNA-sequencing (LCM-seq) developed for single-cell transcriptomics. Fixed cells are subjected to direct lysis without RNA extraction, which both simplifies the experimental procedures as well as lowers technical noise. We apply LCM-seq on neurons isolated from mouse tissues, human post-mortem tissues, and illustrate its utility down to single captured cells. Importantly, we demonstrate that LCM-seq can provide biological insight on highly similar neuronal populations, including motor neurons isolated from different levels of the mouse spinal cord, as well as human midbrain dopamine neurons of the substantia nigra compacta and the ventral tegmental area

Rapid Serial Visual Presentation påmoderna mobiltelefoner

This thesis is a comparative study of Rapid Serial Visual Presentation (RSVP) which is a dynamic method of presenting text. The aim of the study was to investigate how to improve the user friendlyness of RSVP for today's mobile phones and technology. To fulfill the purpose, the aspects of the method which could be improved was investigated and then implemented in a prototype. The prototype was tested in an experimental study after which the result was analyzed and compared with previous studies of RSVP. The new features that were implemented in this study showed no significant improvements in usability compared with previous studies, but proved to be more user-friendly in comparison with normal spatial reading.Denna uppsats är en jämförande studie av Rapid Serial Visual Presentation (RSVP) som är en dynamisk metod för att presentera text. Syftet med studien var att undersöka hur man kan förbättra användarvänligheten för RSVP till dagens mobiltelefoner och teknik. För att uppfylla syftet undersöktes vilka aspekter som kunde förbättras med metoden för att sedan implementera dessa i en prototyp. Prototypen testades i en experimentstudie varefter resultaten analyserades och jämfördes med tidigare studier av RSVP. De nya funktionerna som implementerades i denna studie visade inga märkbara förbättringar i användarvänlighet jämfört med tidigare studier, men visade sig vara mer användarvänliga i jämförelse med den normala spatiala läsningen

Rapid Serial Visual Presentation påmoderna mobiltelefoner

This thesis is a comparative study of Rapid Serial Visual Presentation (RSVP) which is a dynamic method of presenting text. The aim of the study was to investigate how to improve the user friendlyness of RSVP for today's mobile phones and technology. To fulfill the purpose, the aspects of the method which could be improved was investigated and then implemented in a prototype. The prototype was tested in an experimental study after which the result was analyzed and compared with previous studies of RSVP. The new features that were implemented in this study showed no significant improvements in usability compared with previous studies, but proved to be more user-friendly in comparison with normal spatial reading.Denna uppsats är en jämförande studie av Rapid Serial Visual Presentation (RSVP) som är en dynamisk metod för att presentera text. Syftet med studien var att undersöka hur man kan förbättra användarvänligheten för RSVP till dagens mobiltelefoner och teknik. För att uppfylla syftet undersöktes vilka aspekter som kunde förbättras med metoden för att sedan implementera dessa i en prototyp. Prototypen testades i en experimentstudie varefter resultaten analyserades och jämfördes med tidigare studier av RSVP. De nya funktionerna som implementerades i denna studie visade inga märkbara förbättringar i användarvänlighet jämfört med tidigare studier, men visade sig vara mer användarvänliga i jämförelse med den normala spatiala läsningen